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[(2)6.7(7)-.9(,28,3)6.7(942)]TJ
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0 Tc
[(No)-348.7(patient)-344.5(identiers)-353.2(were)-347.4(obtained,)-343.1(and)-350(therefore)-347.5(no)-347.8(Uni-)]TJ
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[(versity)-579.1(of)-572.4(California,)-576.8(Los)-574.5(Angeles)-575.3(\(UCLA\))-575(institutional)]TJ
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[(review)-303.6(board)-306.1(approval)-299.2(was)-304.6(required)-304.9([45)-302.8(CFR)-299(46.102\(f)-86(\)].)-299.3(In)]TJ
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[(brief,)-430.6(human)-422.6(lipoaspirate)-431.7(from)-420.3(6)-427.3(cosmetic)-428.3(liposuction)-422.5(pa-)]TJ
T*
[(tients)-295.9(were)-295.6(collected)-297.2(and)-292.4(digested)-293.4(with)-293.5(collagenase)-294.5(\(Sigma-)]TJ
0 -1.1174 TD
[(Aldrich,)-238(St.)-236(Louis,)-234.7(MO\))-235.4(to)-232.7(get)-235(human)-232.5(SVF.)-237.7(Adipocytes)-238.9(were)]TJ
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[(separated)-406.1(by)-399.6(centrifugation)-405.3(and)-401.9(excluded.)-401.8(Red)-402.9(blood)-403.1(cells)]TJ
25.5097 67.9184 TD
[(were)-243.7(removed)-247.4(by)-244.1(adding)-249.8(a)-244.2(red)-240.6(cell)-248.9(lysis)-247.3(buffer)-243.8(\(eBioscience,)]TJ
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[(San)-419(Diego,)-425.8(CA\))-419.9(to)-422.8(SVF.)-422(The)-426.1(processed)-421.9(SVF)-424.3(was)-419.8(ltered)]TJ
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[(through)-457.1(a)-457.3(70-)]TJ
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(m)Tj
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[(m)-457.3(cell)-462(lter.)-459.4(The)-454.9(resulting)-460.5(hSVF)-460(was)-460.2(im-)]TJ
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[(mediately)-337.6(processed)-335.5(for)-337.3(hPSC)-333.4(purication.)]TJ
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T*
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[(cell)-306.5(population)-306.3(was)-310.4(processed)-306.7(on)-307.5(the)-304.1(uorescence-activated)]TJ
T*
[(cell)-306.5(sorting)-307.2(\(FACS\))-307.6(Aria)-311.8(cell)-306.5(sorter)-307.1(\(BD)-310.4(Biosciences\).)-310.2(4)]TJ
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()Tj
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(,6-)Tj
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[(Diamidino-2-phenylindole)-493.5(was)-489(used)-483.4(for)-487(exclusion)-490.4(of)-486(any)]TJ
0 -1.1116 TD
[(nonviable)-398.4(cells)-399.6(in)-394(cell)-398.6(sorting.)-397.1(Consequently,)-400.2(two)-396(p)-24.1(o)-18.4(p)-18.4(u)-24.1(l)-18.5(a)-19.5(-)]TJ
T*
.02 Tc
[(tions)-372.6(of)-372.7(cells)-372.6(w)-5(e).5(re)-379.7(sorted)-372.7(a)-5.3(ccording)-372.8(t)-4.3(o)-372.8(t)1.5(he)-5.3(ir)-372.7(c)-5.3(e).5(ll)-372.9(surfac)-5.3(e)]TJ
T*
0 Tc
[(markers)-558.2(to)-555.3(constitute)-560.8(hPSCs:)-554.4(distinct)-557.5(pericytes)-560.6(\(CD34)]TJ
/F7 1 Tf
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(-)Tj
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(,)Tj
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(CD146)Tj
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(+)Tj
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[(,)-429.6(CD45)]TJ
/F7 1 Tf
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(-)Tj
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[(\))-427.3(and)-430.7(adventitial)-436.7(cells)-434.1(\(CD34)]TJ
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[(,)-429.6(CD146)]TJ
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(-)Tj
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(,)Tj
-24.041 -1.1116 TD
(CD45)Tj
/F7 1 Tf
2.3845 0 TD
(-)Tj
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(\))Tj
7.3823 0 0 6.5614 343.0487 554.1731 Tm
[(27,39,40,43)-11.3(,44)]TJ
9.843 0 0 9.843 390.1605 549.8077 Tm
[(\(Supplementary)-285.1(Fig.)-277.4(S1;)-279.7(Supplementary)]TJ
-8.0578 -1.1116 TD
[(Data)-336(are)-333.9(available)-337.5(online)-336.3(at)-331.8(www.liebertpub.com/te)-13.9(a\).)]TJ
/F11 1 Tf
8.9663 0 0 8.9663 310.8472 516.9258 Tm
[(Human)-333.8(articular)-337(cartilage)-332.9(sample)-333.8(preparation)]TJ
0 -1.2203 TD
[(and)-329.4(human)-335.4(articular)-337(chondrocyte)-334.2(isolation)]TJ
/F4 1 Tf
9.843 0 0 9.843 320.8251 489.5432 Tm
[(Human)-250.7(articular)-255.6(cartilage)-256.8(samples)-252.9(were)-255.2(obtained)-253.3(from)-253.2(six)]TJ
-1.0137 -1.1174 TD
[(knee)-409.9(replacement)-411.1(patients.)-412.2(No)-406.3(patient)-413.6(identiers)-410.8(were)-410.7(ob-)]TJ
0 -1.1116 TD
[(tained,)-387(and)-384.6(therefore)-387.8(no)-382.3(University)-387.6(of)-382.3(California,)-392.5(Los)-384.4(An-)]TJ
T*
[(geles,)-345.3(institutional)-350.3(review)-349.7(board)-352.2(approval)-345.3(was)-350.7(required)-351([45)]TJ
T*
[(CFR)-298.9(46.102\(f)-86(\)].)-299.3(The)-299.4(samples)-304.8(were)-301.3(xed)-299.2(in)-301.8(4%)-297(paraformal-)]TJ
0 -1.1174 TD
[(dehyde)-487(\(PFA,)-480.6(Sigma-Aldrich\))-492.3(at)-481.5(4)]TJ
/F7 1 Tf
14.7564 0 TD
()Tj
/F4 1 Tf
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[(C)-480.2(overnight)-489.4(and)-482.5(dec-)]TJ
-15.1538 -1.1116 TD
[(alcied)-397.2(with)-391.4(19%)-396(ethylenediaminetetraacetic)-406.5(acid)-398.5(\(EDTA;)]TJ
T*
[(Sigma-Aldrich\))-336.8(for)-337.3(2)-329.4(weeks)-336.8(before)-337.2(parafn)-338.1(embedding.)]TJ
1.0137 -1.1174 TD
[(Hu)20(ma)18.4(n)-237.2(a)0(r)19.7(t)0(i)20.6(c)0(u)19.7(l)0(a)19.6(r)-243(ch)19.7(on)20.8(dr)20.9(oc)19.7(yt)20.7(es)-233.6(\(h)20.9(AR)18.9(Cs)20(\))-237.2(w)0(e)18.8(r)0(e)-233.7(i)0(s)20.9(o)0(l)20.7(a)0(t)19.5(e)0(d)-228(f)0(r)15.2(o)0(m)]TJ
-1.0137 -1.1116 TD
[(pooled)-480.2(femoral)-483.6(and)-476.8(tibial)-480.6(articular)-486(cartilage)-481.5(with)-483.6(healthy)]TJ
T*
[(appearance)-352(from)-345.4(six)-348.8(knee)-346.6(replacement)-353.5(patients)-351.2(by)-347.8(incubat-)]TJ
0 -1.1174 TD
[(ing)-326(with)-333.8(1)-162.4(mg/mL)-331.8(trypsin)-330.3(\(Sigma-Aldrich\))-332.1(for)-331.5(1)-168.1(h)-329.4(followed)]TJ
0 -1.1116 TD
[(by)-290.2(an)-291.3(overnight)-293.5(digestion)-293.5(in)-290.3(0.5)-166.9(mg/mL)-297.3(type)-293.7(II)-290.1(collagenase.)]TJ
T*
[(The)-587.4(following)-592.9(morning,)-594.2(the)-592.1(isolated)-593.1(chondrocytes)-592.6(were)]TJ
0 -1.1174 TD
[(washed)-274.6(with)-276.2(a)-273(complete)-278.7(medium)-275.3(\()-81.7(MEM-)]TJ
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16.7089 0 TD
(a)Tj
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(+)Tj
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[(10%)-275.1(FBS\))-275.8(and)]TJ
-18.3562 -1.1116 TD
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/F7 1 Tf
10.4596 0 TD
()Tj
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(10)Tj
7.3823 0 0 6.5614 430.4125 329.4991 Tm
(4)Tj
9.843 0 0 9.843 437.1022 325.1338 Tm
[(ce)18.5(ll)14.8(s/)20.9(mm)]TJ
7.3823 0 0 6.5614 472.6487 329.4991 Tm
(2)Tj
9.843 0 0 9.843 479.3384 325.1338 Tm
[(fo)20.9(r)-312.1(c)0(u)19.7(l)0(t)20.6(u)0(r)20.9(e)-307.5(ex)19.7(pa)19.7(n-)]TJ
-17.1178 -1.1116 TD
[(si)20.9(on)20.8(.)-291.4(T)0(h)19.6(e)-290.2(me)18.4(di)14.9(um)-279.9(wa)18.8(s)-288.9(c)0(h)13.9(a)0(n)19.7(g)0(e)19.7(d)-289.1(ev)19.7(er)19.7(y)-294.8(3)-289.1(da)19.7(ys)21(.)-291.4(A)0(f)14.2(t)0(e)19.6(r)-289.1(re)19.7(ac)18.5(hi)20.7(ng)]TJ
T*
[(70)20.8(%)-405.3(c)0(o)19.7(n)0()21(u)0(e)19.7(n)0(c)19.7(e)0(,)-397.4(c)0(e)18.5(l)0(l)20.6(s)-404.1(we)13.1(re)-395(us)21(ed)-395(fo)20.9(r)-404.2(R)0(N)18.9(A)-405.1(is)15.1(ol)20.7(at)19.5(io)20.7(n)-404.3(o)0(r)-393.8(i)0(m)19.5(-)]TJ
0 -1.1174 TD
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/F11 1 Tf
8.9663 0 0 8.9663 310.8472 270.3684 Tm
[(Induction)-335.5(of)]TJ
/F2 1 Tf
5.5389 0 TD
[(in)-336.5(vitro)]TJ
/F11 1 Tf
3.3259 0 TD
(chondrogenesis)Tj
/F4 1 Tf
9.843 0 0 9.843 320.8251 253.9275 Tm
[(Human)-337.1(BMSCs)-342.2(were)-335.9(purchased)-341.5(from)-339.6(Promo)-340.6(Cell)-335.2(\(Cata-)]TJ
-1.0137 -1.1174 TD
[(log)-331.7(Number:)-339.4(C-12975,)-334.7(Lot)-332.9(Number:)-333.6(1080401.3\).)]TJ
1.0137 -1.1116 TD
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-1.0137 -1.1116 TD
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0 -1.1174 TD
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(+)Tj
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[(1%)-461.9(I)5.8(T)-1.2(S)]TJ
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[(10)-5.7(0)]TJ
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(50)Tj
/F6 1 Tf
1.1865 0 TD
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(m)Tj
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[(g)6.5(/).7(m)5.3(L)-380.5(as)6.7(cor)6.6(b).8(i)6.4(c)-380.5(ac)5.4(id)]TJ
/F7 1 Tf
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[(1m)160.1(M)]TJ
-21.9387 -1.1116 TD
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[(s)5.5(odi)5.2(um)-318.4(p)5.3(y)-.4(r)5.4(uvate)]TJ
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(40)Tj
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[(de)-6.9(ns)-5.5(i)-5.8(t)-.1(y)-276.4(o)-5.7(f)-270.6(1)]TJ
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()Tj
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(10)Tj
7.3823 0 0 6.5614 377.6314 170.5889 Tm
0 Tc
(6)Tj
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[(cells)5.4(/mL.)-267.8(Sp)5.2(heroi)5.1(ds)-265.3(of)-271.2(3)]TJ
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()Tj
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(10)Tj
7.3823 0 0 6.5614 502.6959 170.5889 Tm
0 Tc
(5)Tj
9.843 0 0 9.843 509.1589 166.2236 Tm
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[(ce)-4.9(lls)-274.2(w)1.2(e)-4.9(r)-3.7(e)]TJ
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[(f)5.5(ormed)-438(by)-443.8(cent)5.3(ri)5.3(fug)5.4(in)5.4(g)-443.8(3)-.3(0)5.4(0)]TJ
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(b)Tj
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[(32,)-17(33,)-17(4)3.4(5)]TJ
9.843 0 0 9.843 488.8062 122.3999 Tm
0 Tc
[(an)19.7(d)-398.5(t)0(h)20.7(e)-393.9(gr)20.9(ow)20(th)]TJ
-18.0797 -1.1116 TD
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10.1371 0 TD
(m)Tj
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T*
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[(\(2000)-166.2(ng/mL\))]TJ
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[(\(10)-164.2(ng/mL\))]TJ
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[(\(25)-164.2(ng/mL\))]TJ
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T*
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[(when)-593.9(compared)-589.5(to)-589.8(hPSCs)-593.5(\(Fig.)-589.5(2B)-590.7(and)-586.2(Supplementary)]TJ
T*
[(Table)-354.7(S3-1\).)-354.3(As)-354.2(hPSCs)-357.4(underwent)-353(chondrogenesis)-358.5(in)-353.7(basic)]TJ
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[(chondrogenic)-420(media)-418.1(for)-417.9(a)-417(total)-416(of)-416.9(8)-415.8(weeks)-417.5(\(56)-418(days\),)-413.1(the)]TJ
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(NELL-1)Tj
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[(cartilage)-674.8(oligomeric)-671(matrix)-671.1(protein)]TJ
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[(,)-665.8(Fig.)-669.1(2D\),)]TJ
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[(COLLAGEN)-331.5(II)]TJ
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[(\(Fig.)-324.6(2E\),)-324.7(and)]TJ
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[(\(Fig.)-324.6(2F\))-325.7(in)-319.1(the)]TJ
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25.5097 16.6974 TD
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[(Recombinant)-334.5(human)-335.5(NELL-1)-332.2(has)-330.8(prochondrogenic)]TJ
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[(effects)-334.6(on)-331.3(hPSCs)]TJ
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[(List)-333.4(of)-331.2(Primers)-336(for)-332.2(Quantitative)-337.3(Real-Time)-334.5(Polymerase)-338.3(Chain)-330.8(Reaction)]TJ
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[(Neural)-337.5(EGFL)-335.2(like)-333.7(1)-335(\(NELL1\))-17396.3(5)]TJ
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30.1978 0 TD
()Tj
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[(-TAT)-335.4(GAG)-339.4(CGT)-334.2(GTG)-334(ATA)-334(GAC)-339.6(CCT)-334.4(C-3)]TJ
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18.4273 0 TD
()Tj
/F4 1 Tf
-19.176 -1.0135 TD
(5)Tj
/F7 1 Tf
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()Tj
/F4 1 Tf
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[(-TCC)-335.4(CAT)-340(CTT)-334.5(GGA)-333.7(TGA)-334(TCC)-334.4(CTT-3)]TJ
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17.0626 0 TD
()Tj
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-47.508 -1.2151 TD
[(SRY)-338.6(\(sex)-333.2(determining)-337.4(region)-335.5(Y\)-box)-335(9)-329.2(\(SOX9\))-10136.7(5)]TJ
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30.1978 0 TD
()Tj
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[(-AGC)-340.6(GAA)-333.7(CGC)-334(ACA)-333.8(TCA)-334.2(AGA)-339.4(C-3)]TJ
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()Tj
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(5)Tj
/F7 1 Tf
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()Tj
/F4 1 Tf
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[(-CTG)-335.2(TAG)-339.8(GCG)-333.8(ATC)-334.2(TGT)-334.4(TGG)-334(GG-3)]TJ
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()Tj
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[(Collagen,)-337.5(type)-333.5(II,)-333.6(alpha)-335.7(1)-335(\(COL2\))-15666.4(5)]TJ
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()Tj
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[(-TCC)-335.4(TCT)-334.5(GCG)-339.6(ACG)-333.9(ACA)-333.9(TAA)-334(TC-3)]TJ
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()Tj
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(5)Tj
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()Tj
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[(-GAG)-340.4(GTC)-334.2(AGT)-334(TGG)-334(GCA)-333.8(GAT)-339.8(G-3)]TJ
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16.3024 0 TD
()Tj
/F4 1 Tf
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[(Aggrecan)-340.5(\(AGG\))-22644.3(5)]TJ
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30.1978 0 TD
()Tj
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[(-AAC)-340.6(ATC)-334.2(AGG)-333.7(AGT)-334(CCC)-334.2(TGA)-339.8(CC-3)]TJ
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16.8668 0 TD
()Tj
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-17.6154 -1.0135 TD
(5)Tj
/F7 1 Tf
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()Tj
/F4 1 Tf
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[(-CCA)-335(CTT)-340.3(CAA)-333.9(CCA)-334(AAC)-333.8(TGG)-334(TG-3)]TJ
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16.7631 0 TD
()Tj
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[(Cartilage)-342.2(oligomeric)-336.4(matrix)-331.1(protein)-336.7(\(COMP\))-11364.2(5)]TJ
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30.1978 0 TD
()Tj
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[(-CAT)-335.2(CAG)-339.6(GGT)-334(GCG)-333.9(ATT)-334.4(CTA)-334.2(TG-3)]TJ
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16.7113 0 TD
()Tj
/F4 1 Tf
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(5)Tj
/F7 1 Tf
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()Tj
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[(-TCC)-335.4(TGG)-339.8(GAG)-333.7(AAG)-333.7(CAG)-333.8(AAG)-339.4(AC-3)]TJ
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17.1259 0 TD
()Tj
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[(Collagen,)-337.5(type)-333.5(X,)-333.4(alpha)-335.7(1)-335(\(COL10\))-15114.6(5)]TJ
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()Tj
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[(-CAG)-340.6(GCA)-333.9(ACA)-333.8(GCA)-333.8(TTA)-334.4(TGA)-339.8(CC-3)]TJ
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()Tj
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(5)Tj
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()Tj
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[(-AGG)-340.4(CCT)-334.4(ACC)-334(CAA)-333.9(ACA)-333.8(TGA)-339.8(GT-3)]TJ
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[(Matrix)-336.5(metallopeptidase)-340.3(13)-336(\()-81.6(MMP13\))-14081.3(5)]TJ
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()Tj
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[(-TGA)-335(CCC)-340(TTC)-334.5(CTT)-334.5(ATC)-334.2(CCT)-334.4(TG-3)]TJ
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(5)Tj
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()Tj
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[(-GCA)-340.6(TCA)-334.2(ACC)-334(TGC)-334.2(TGA)-334(GGA)-339.4(T-3)]TJ
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()Tj
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[(ADAM)-289.4(metallopeptidase)-294.2(with)-287.3(thrombospondin)-291.6(type)-287.5(1motif,)]TJ
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[(4)-329.2(\(ADAMTS4\))]TJ
28.7006 1.0135 TD
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()Tj
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[(-TTT)-335.7(GAC)-339.6(AAG)-333.7(TGC)-334.2(ATG)-334(GTG)-334(TG-3)]TJ
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[(-TCC)-335.4(GTA)-339.8(CCT)-334.4(GAA)-333.7(TTT)-334.7(CCT)-334.4(GA-3)]TJ
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()Tj
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[(Collagen,)-337.5(type)-333.5(I,)-332.6(alpha)-335.7(1)-335(\(COL1\))-16000.4(5)]TJ
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()Tj
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[(-CAG)-340.6(CCG)-334(CTT)-334.5(CAC)-334(CTA)-334.2(CAG)-333.8(C-3)]TJ
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16.0433 0 TD
()Tj
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-16.7919 -1.0135 TD
(5)Tj
/F7 1 Tf
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()Tj
/F4 1 Tf
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[(-TTT)-335.7(TGT)-334.4(ATT)-340.1(CAA)-333.9(TCA)-334.2(CTG)-334.2(TCG)-334.2(CC-3)]TJ
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18.6807 0 TD
()Tj
/F4 1 Tf
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[(Osteocalcin)-339.2(\(OCN\))-21855.5(5)]TJ
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30.1978 0 TD
()Tj
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[(-GGC)-340.6(GCT)-334.2(ACC)-334(TGT)-334.4(ATC)-334.2(AAT)-334(GG-3)]TJ
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()Tj
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(5)Tj
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()Tj
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[(-GTG)-335(GTC)-340(AGC)-333.8(CAA)-333.9(CTC)-334.4(GTC)-334.2(A-3)]TJ
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16.0951 0 TD
()Tj
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[(Osteopontin)-335.7(\(OPN\))-21814(5)]TJ
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30.1978 0 TD
()Tj
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[(-GAA)-340.4(GTT)-334.4(TCG)-334.2(CAG)-333.8(ACC)-334(TGA)-339.8(CAT-3)]TJ
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17.4829 0 TD
()Tj
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(5)Tj
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()Tj
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9.845 0 0 9.845 359.4897 436.8755 Tm
[(-GTA)-335(TGC)-340(ACC)-334(ATT)-334.4(CAA)-333.9(CTC)-334.4(CTC)-334.4(G-3)]TJ
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18.2719 0 TD
()Tj
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[(Vascular)-340.5(endothelial)-337.6(growth)-335.2(factor)-330.9(A)-335.8(\(VEGF\))-10834.6(5)]TJ
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()Tj
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[(-AGG)-340.4(GCA)-333.8(GAA)-333.7(TCA)-334.2(TCA)-334.2(CGA)-339.6(AGT-3)]TJ
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()Tj
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(5)Tj
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()Tj
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[(-AGG)-340.4(GTC)-334.2(TCG)-334.2(ATT)-334.4(GGA)-333.7(TGG)-339.8(CA-3)]TJ
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16.8668 0 TD
()Tj
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[(Marker)-337.3(of)-336(proliferation)-332.6(Ki-67)-339.9(\()-81.6(MKI67\))-13540.2(5)]TJ
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()Tj
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[(-GCC)-335(TGC)-340(TCG)-334.2(ACC)-334(CTA)-334.2(CAG)-333.8(A-3)]TJ
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()Tj
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(5)Tj
/F7 1 Tf
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()Tj
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[(-GCT)-335.2(TGT)-340.1(CAA)-333.9(CTG)-334.2(CGG)-333.9(TTG)-334.4(C-3)]TJ
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15.9397 0 TD
()Tj
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[(Proliferating)-338.2(cell)-334.9(nuclear)-338.9(antigen)-332.1(\(PCNA\))-12596.6(5)]TJ
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30.1978 0 TD
()Tj
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[(-GCG)-340.6(TGA)-334(ACC)-334(TCA)-334.2(CCA)-334(GTA)-339.8(TGT-3)]TJ
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()Tj
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(5)Tj
/F7 1 Tf
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()Tj
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[(-TCT)-335.5(TCG)-340(GCC)-334(CTT)-334.5(AGT)-334(GTA)-334(ATG)-334(AT-3)]TJ
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18.8362 0 TD
()Tj
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[(Caspase)-339.3(3,)-332.6(apoptosis-related)-338.6(cysteine)-339.8(peptidase)-335.1(\(CASP3\))-6406.1(5)]TJ
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30.1978 0 TD
()Tj
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[(-AGA)-340.4(GGG)-333.7(GAT)-334(CGT)-334.2(TGT)-334.4(AGA)-339.4(AGT)-334(C-3)]TJ
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()Tj
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(5)Tj
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()Tj
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[(-ACA)-340.6(GTC)-334.2(CAG)-333.9(TTC)-334.5(TGT)-334.4(ACC)-334(ACG-3)]TJ
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()Tj
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[(Bone)-334.4(morphogenetic)-340.4(protein)-336.7(receptor)-334.1(Type)-334.5(IA)-336.8(\(BMPRIA\))-5821.7(5)]TJ
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()Tj
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[(-AGA)-340.4(TGA)-334(CCA)-334(GGG)-333.7(AGA)-339.4(AAC)-333.9(CAC-3)]TJ
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[(-CAA)-340.6(CAT)-334.2(TCT)-334.5(ATT)-334.4(GTC)-334.2(CGG)-333.9(CGT)-334.2(A-3)]TJ
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()Tj
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[(Bone)-334.4(morphogenetic)-340.4(protein)-336.7(receptor)-334.1(Type)-334.5(IB)-337(\(BMPRIB\))-5925.6(5)]TJ
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30.1978 0 TD
()Tj
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[(-ACC)-335(AGA)-339.4(CCT)-334.4(CGA)-333.9(TAC)-334.2(AGC)-339.6(ATT-3)]TJ
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(5)Tj
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()Tj
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[(-CCC)-335.2(ATA)-339.8(GCG)-333.8(ACC)-334(TTT)-334.7(TCC)-334.4(AAT-3)]TJ
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()Tj
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[(Bone)-334.4(morphogenetic)-340.4(protein)-336.7(receptor)-334.1(Type)-334.5(II)-336(\(BMPRII\))-6592.6(5)]TJ
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()Tj
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[(-GAC)-340.6(AGG)-333.7(AGA)-333.7(CCG)-334(TAA)-339.8(ACA)-333.9(AGG-3)]TJ
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(5)Tj
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()Tj
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[(-CCA)-335(TAT)-340.1(CGA)-333.8(CCT)-334.4(CGG)-333.9(CCA)-334(ATC-3)]TJ
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()Tj
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[(Transforming)-337.9(growth)-335.2(factor)]TJ
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(b)Tj
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[(receptor)-334.1(I)-335(\(TGF)]TJ
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(b)Tj
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[(RI\))-8728.2(5)]TJ
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()Tj
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[(-GCT)-335.2(GTA)-339.8(TTG)-334.4(CAG)-333.9(ACT)-334.2(TAG)-334(GAC)-339.6(TG-3)]TJ
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()Tj
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(5)Tj
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()Tj
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[(-TTT)-335.7(TTG)-334.4(TTC)-340.3(CCA)-334(CTC)-334.4(TGT)-334.4(GGT)-334(T-3)]TJ
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()Tj
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8.9663 0 0 8.9663 59.7543 749.7069 Tm
[(NELL-1)-341(ACCELERATED)-338.1(HPSC)]TJ
6.4556 0 0 6.4556 189.581 749.7069 Tm
(S)Tj
8.9663 0 0 8.9663 196.8944 749.7069 Tm
[(CHONDROGENIC)-333.8(DIFFERENTIATION)-20064.5(275)]TJ
ET
endstream
endobj
103 0 obj
<>stream
/GS1 gs
q
489.77 0 0 111.118 62.192 621.127 cm
/Im1 Do
Q
BT
/F9 1 Tf
9.845 0 0 9.845 62.022 604.3463 Tm
0 0 0 rg
0 Tc
0 Tw
[(FIG.)-463.4(1.)]TJ
/F4 1 Tf
4.244 0 TD
[(NEL-like)-461.8(molecule-1)-464.1(\(NELL-1\))-467.2(immunostaining)-461.3(in)-462.8(human)-462.2(articular)-467.9(cartilage.)-466.7(NELL-1)-465.2(is)-456.9(mainly)-463.6(present)-464.2(in)]TJ
-4.244 -1.0135 TD
[(chondrocytes)-240(of)-238.1(the)-240.4(supercial)-243.8(\(S\))-238.9(and)-234.5(middle)-244.7(\()-81.6(M)0(\))-238.9(zones)-237.5(\()]TJ
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23.4315 0 TD
[(red)-240.1(box)]TJ
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[(\))-242.9(and)-234.5(in)-238.3(the)-240.4(uncalcied)-245.1(\(UC\))-236.1(rather)-244.6(than)-235.6(calcied)-243.1(\(C\))-240.1(deep)]TJ
-26.449 -1.0135 TD
[(\(D\))-337.8(zone)-334.5(chondrocytes)-332.1(\()]TJ
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9.9565 0 TD
[(blue)-333.5(box)]TJ
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[(\).)-332.6(Color)-334.4(images)-337.7(available)-336.6(online)-335.7(at)-331.6(www.liebertpub.com/tea)]TJ
ET
q
420.92 0 0 378.822 96.775 153.014 cm
/Im2 Do
Q
BT
/F9 1 Tf
9.845 0 0 9.845 62.022 136.2897 Tm
[(FIG.)-313.7(2.)]TJ
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[(Th)19.9(e)-307.4(e)0(x)19.9(p)0(r)21.1(e)0(s)20.1(s)0(i)15.3(o)0(n)-289.9(l)0(e)13.9(v)0(e)19.9(l)-300.6(of)-295.7(NE)19.1(LL)18.7(-1)-295.7(is)-295.7(po)21.1(si)21(ti)20.7(ve)19.9(ly)-295.8(re)19.9(la)19.7(te)19.7(d)-306.2(t)0(o)-295.8(c)0(h)19.9(o)0(n)21.1(d)0(r)21.1(o)0(g)21.1(e)0(n)19.9(e)0(s)14.3(i)0(s)21(.)]TJ
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[(\(A)20.2(\))]TJ
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[(Ge)19.1(ne)-296.8(ex)19.9(pr)21.1(es)20(si)15.3(on)-289.9(le)19.7(ve)14.1(ls)-289.9(of)]TJ
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[(NE)18.9(LL)21.4(-1)]TJ
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(in)Tj
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36.1809 0 TD
()Tj
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[(SD)-336.6(of)-336(ce)18.7(ll)20.7(s)-346.3(f)0(r)21(o)0(m)-337.2(s)0(i)21(x)-346.5(do)21(no)21.1(rs)-335.8(ar)19.9(e)]TJ
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[(sh)21.2(ow)20.2(n.)-257.7(*)]TJ
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(p)Tj
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(<)Tj
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[(0.)18.7(05)-255.4(ve)19.9(rs)21.2(us)-255.2(AR)19.2(C)]TJ
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7.0485 0 TD
[(NE)18.9(LL)21.4(-1)]TJ
/F4 1 Tf
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[(ex)19.9(pr)15.3(es)20.1(si)21(on)21.1(.)]TJ
/F9 1 Tf
4.699 0 TD
[(\(B)20.1(\))]TJ
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[(Ex)19.9(pr)21.1(es)20(si)21(on)-255.4(of)-255.4(NE)19.1(LL)18.7(-1)-255.4(in)-255.5(hu)21.1(ma)18.7(n)-265.9(P)0(S)21.4(C)0(s)-256.2(a)0(n)19.9(d)-265.9(hu)21.1(ma)18.7(n)-265.9(A)0(R)19.2(C)0(s)-256.2(e)0(x)19.9(a)0(m)18.7(i)0(n)20.9(e)0(d)-256.5(b)0(y)]TJ
-21.6809 -1.0077 TD
[(im)19.7(mu)19.9(no)21.1(cy)19.9(to)20.9(ch)14.1(em)24.5(ic)13.9(al)-354.6(st)21(ai)19.7(ni)20.9(ng)21.1(.)-366.2(S)0(c)20.1(a)0(l)19.7(e)-365(ba)19.9(r)]TJ
/F7 1 Tf
16.527 0 TD
(=)Tj
/F4 1 Tf
.7141 0 TD
(50)Tj
/F6 1 Tf
1.146 0 TD
(m)Tj
/F4 1 Tf
.5413 0 TD
[(m.)-351(mR)18.9(NA)-354.9(le)19.7(ve)14.1(ls)-353.3(of)]TJ
/F5 1 Tf
8.4478 0 TD
[(NE)18.9(LL)21.4(-1)]TJ
/F9 1 Tf
3.53 0 TD
[(\(C)14.5(\))]TJ
/F4 1 Tf
1.359 0 TD
(,)Tj
/F5 1 Tf
.6104 0 TD
[(CO)19.2(MP)]TJ
/F9 1 Tf
3.1672 0 TD
[(\(D)14.5(\))]TJ
/F4 1 Tf
1.359 0 TD
(,)Tj
/F5 1 Tf
.6104 0 TD
[(CO)19.2(LL)15.6(AG)19.1(EN)-355.4(II)]TJ
/F9 1 Tf
6.4208 0 TD
[(\(E)20.1(\),)]TJ
/F4 1 Tf
1.9176 0 TD
[(an)19.9(d)]TJ
/F5 1 Tf
1.7909 0 TD
[(AG)19.1(-)]TJ
-48.1414 -1.0135 TD
[(GR)19.1(EC)18.9(AN)]TJ
/F9 1 Tf
4.1634 0 TD
[(\(F)19.9(\))]TJ
/F4 1 Tf
1.5836 0 TD
[(in)-307.4(ch)19.9(on)21.1(dr)15.3(og)21.1(en)19.9(ic)-308.5(di)20.9(ff)15.3(er)19.9(en)19.9(ti)20.7(at)19.7(ed)-308.4(hP)15.5(SC)20.2(s)-317.5(a)0(t)-308.5(d)0(i)15.1(f)0(f)21(e)0(r)19.9(e)0(n)19.9(t)-317.9(ti)14.9(m)15.1(e)-324.6(po)21.1(in)20.9(ts)21(.)-320.1(M)0(e)19.1(a)0(n)]TJ
/F7 1 Tf
27.4625 0 TD
()Tj
/F4 1 Tf
.7141 0 TD
[(SD)-307.8(of)-307.2(ce)18.7(ll)14.9(s)-317.5(f)0(r)21(o)0(m)-308.4(s)0(i)15.3(x)-317.7(do)21.1(no)21.1(rs)-312.8(ar)19.9(e)-318.9(s)0(h)21.2(o)0(w)14.5(n)0(.)]TJ
-33.9236 -1.0135 TD
(*)Tj
/F5 1 Tf
.4895 0 TD
(p)Tj
/F7 1 Tf
.6565 0 TD
(<)Tj
/F4 1 Tf
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[(0.)18.7(05)-249.6(ve)19.9(rs)15.5(us)-249.4(ex)19.9(pr)21(es)20.1(si)15.3(on)-249.6(le)19.7(ve)19.9(l)-260.3(a)0(t)-256.7(w)0(e)19.1(e)0(k)-250.8(0)0(,)-252(*)0(*)]TJ
/F5 1 Tf
16.6825 0 TD
(p)Tj
/F7 1 Tf
.6565 0 TD
(<)Tj
/F4 1 Tf
.7083 0 TD
[(0.)18.7(01)-249.6(ve)19.9(rs)21.2(us)-255.2(ex)19.9(pr)21.1(es)20(si)21(on)-255.4(le)19.7(ve)19.9(l)-260.3(a)0(t)-250.9(w)0(e)19.1(e)0(k)-256.5(0)0(.)-246.2(T)0(h)19.9(e)-267.1(co)19.9(rr)21(el)19.7(at)19.7(io)20.9(n)-265.9(b)0(e)19.9(t)0(w)20.1(e)0(e)18.7(n)-260.1(th)20.9(e)-261.3(m)0(R)18.9(N)0(A)]TJ
-19.9015 -1.0135 TD
[(le)19.7(ve)19.9(l)-248.8(o)0(f)]TJ
/F5 1 Tf
3.2305 0 TD
[(NE)18.9(LL)15.6(-1)]TJ
/F4 1 Tf
3.4148 0 TD
[(an)19.9(d)-248.6(t)0(h)20.9(e)-244(mR)18.9(NA)-239.7(le)19.7(ve)19.9(ls)-238.1(of)]TJ
/F5 1 Tf
9.8356 0 TD
[(CO)19.2(MP)]TJ
/F9 1 Tf
3.052 0 TD
[(\(G)19.9(\))]TJ
/F4 1 Tf
1.4166 0 TD
(,)Tj
/F5 1 Tf
.4952 0 TD
[(CO)19.2(LL)21.4(AG)19.1(EN)-240.2(II)]TJ
/F9 1 Tf
6.1789 0 TD
[(\(H)19.9(\),)]TJ
/F4 1 Tf
1.9176 0 TD
[(an)19.9(d)]TJ
/F5 1 Tf
1.67 0 TD
[(AG)19.1(GR)19.1(EC)18.9(AN)]TJ
/F9 1 Tf
5.4073 0 TD
[(\(I)21.2(\))]TJ
/F4 1 Tf
1.2726 0 TD
[(in)-238.3(ch)19.9(on)21(dr)21.1(og)15.3(en)19.9(ic)-233.7(di)20.9(ff)15.3(er)19.9(en)19.9(ti)20.7(at)19.7(ed)]TJ
-37.8912 -1.0077 TD
[(hP)21.2(SC)20.2(s)-231.2(a)0(n)19.9(a)0(l)19.7(y)0(z)14.1(e)0(d)-216.2(b)0(y)-220.8(t)0(h)20.9(e)-232.5(Pe)20.1(ar)19.9(so)21.2(n)-231.3(c)0(o)19.9(r)0(r)15.3(e)0(l)19.7(a)0(t)19.7(i)0(o)20.9(n)-231.3(te)19.7(st)21(.)-228(*)0(P)21.2(o)0(s)15.5(i)0(t)20.7(i)0(v)20.9(e)-232.5(co)19.9(rr)21(el)19.7(at)19.7(io)20.9(n)-231.3(i)0(s)-220.8(s)0(i)21(g)0(n)21.1(i)0()15.3(c)0(a)18.7(n)0(t)-221(a)0(t)-216.4(t)0(h)20.9(e)-232.5(0.)18.7(00)21.1(1)-231.3(l)0(e)19.7(v)0(e)19.9(l)-231.5(\(t)20.9(wo)20.2(-t)20.9(ai)19.7(le)19.7(d\))15.3(.)-228(C)0(o)20.1(l)0(o)20.9(r)-231.3(im)19.7(ag)19.9(es)]TJ
0 -1.0135 TD
[(av)19.9(ai)19.7(la)19.7(bl)15.1(e)-318.9(o)0(n)15.3(l)0(i)20.7(n)0(e)-314.1(a)0(t)-314.3(w)0(w)19.4(w)0(.)17.8(l)0(i)20.7(e)0(b)19.9(e)0(r)19.9(t)0(p)20.9(u)0(b)21.1(.)0(c)17.5(o)0(m)19.9(/)0(t)20.7(e)0(a)]TJ
/F8 1 Tf
8.9663 0 0 8.9663 62.022 749.6502 Tm
(276)Tj
50.2102 0 TD
[(LI)-331.6(ET)-338.7(AL.)]TJ
ET
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